Affinity labeling - download pdf or read online

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121819469

ISBN-13: 9780121819460

The severely acclaimed laboratory regular, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this day - actually a vital booklet for researchers in all fields of existence sciences

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Am. Assoc. Cancer Res. 8, 25 (1967). See also this volume [47]. 36 GENERAL METHODOLOGY ~] DONV does not affect this enzyme. This is as expected, since DONV should not be a substrate for the enzyme. Both azaserine and DON irreversibly inhibit formylglycinamide ribotide amidotransferase. 12 Again, enzymic protonation precedes inactivation. In general, acid proteases seem to be quite sensitive to inhibition by diazo compounds, although cupric ion may be required for a facile reaction. 1~ Recently, fl-galactosidase has been reported to be irreversibly inhibited by the diazo sugar 2,6-anhydro-l-diazo-l-deoxy-D-glycero-L-manno-heptitel.

Lienhard, Biochemistry 10, 2477 (1971). de inhibitors that may form adducts resembling tetrahedral intermediates in substrate hydrolysis, vary markedly but in parallel as the structure of the substrate (or inhibitor) is varied at points distant from the scissile bondJ ~ Cases of this kind are especially interesting in that they seem to imply the occurrence of conformation changes in the enzyme and/or the substrate during the catalytic process. In principle, such changes can be examined directly with transition-state analogs, using exact structural methods to compare atomic coordinates in complexes corresponding to various stages in the catalytic process.

36 GENERAL METHODOLOGY ~] DONV does not affect this enzyme. This is as expected, since DONV should not be a substrate for the enzyme. Both azaserine and DON irreversibly inhibit formylglycinamide ribotide amidotransferase. 12 Again, enzymic protonation precedes inactivation. In general, acid proteases seem to be quite sensitive to inhibition by diazo compounds, although cupric ion may be required for a facile reaction. 1~ Recently, fl-galactosidase has been reported to be irreversibly inhibited by the diazo sugar 2,6-anhydro-l-diazo-l-deoxy-D-glycero-L-manno-heptitel.

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Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek


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