Get Biomembranes - Part D: Biological Oxidations PDF

By Sidney Fleischer, Lester Packer

ISBN-10: 0076687953

ISBN-13: 9780076687954

ISBN-10: 0121819531

ISBN-13: 9780121819538

The severely acclaimed laboratory commonplace, Methods in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate at the present time - really a necessary e-book for researchers in all fields of lifestyles sciences

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Extra resources for Biomembranes - Part D: Biological Oxidations

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25 M sucrose. The suspension is transferred to a large beaker and placed in a 38 ° water bath for 15 rain. 6 mg of cholate per milligram of protein, and the incubation is continued for an additional 15 rain. The beaker is transferred to an ice bath and step 3 is commenced immediately. Step 3. Ammonium Sulfate Fractionation. 4 g per 100 ml of the suspension. The mixture is stirred until the salt has dissolved and the temperature has dropped to 5 °. All subsequent steps are carried out at 0°-5 ° unless otherwise noted.

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Biol. Chem. 235, 1521 (1960). ,9 H. C. Davies, L. Smith, and R. Wasserman, Biochim. Biophys. Acta 85, 238 (1964). [8] PREPARATION AND PROPERTIES OF COMPLEX IV 45 monoxide, cyanide, azide, hydroxylamine, and sodium sulfide. ~9 Assay Methods Principle. Cytochrome c oxidase is most conveniently assayed by the spectrophotometric method of Smith. 2° The rate of oxidation of ferrocytochrome c is measured by following the decrease in the absorbance of its a band (550 nm) for concentrations of ferrocytochrome c between 1 /zM and 60 /zM, /3 band (521 nm) for concentrations greater than 60 /xM, and y band (418 nm) for concentrations less than 1 /zM.

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Biomembranes - Part D: Biological Oxidations by Sidney Fleischer, Lester Packer

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