By Victor Ginsburg (Eds.)
The seriously acclaimed laboratory ordinary, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really an important ebook for researchers in all fields of lifestyles sciences
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Extra info for Complex Carbohydrates Part E
Reductive Cleavage with Et3SiH and BF3" Et20. A 5-mg sample of the per-O-methylated polysaccharide and a small stirring bar were added to a V-vial, and the contents were dried as described before. 25 ml, predried with Call2), EbSiH (20/A), and redistilled BF3" Et20 (15/zl) were sequentially added, and the vial was capped. 25 ml) was added, and the mixture was deionized by adding Dowex AG 501-X8-D resin, a few beads at a time, until the blue color of the resin was retained. The resin was removed by filtration and washed with MeOH ( - 3 ml), and the filtrate was collected in a 5-ml, round-bottom flask.
Fernandes, and T. W. Rademacher, FEBS Lett. 164, 231 (1983). z7 R. Geyer, H. Geyer, S. Stirm, G. Hunsmann, J. Schneider, U. Dabrowski, and J. Dabrowski, Biochemistry 23, 5628 (1984). 18 A. Yamada, J. Dabrowski, P. Hanfland, and H. Egge, Biochim. Biophys. Acta 618, 473 (1980). t9 j. Dabrowski and P. Hanfland, FEBS Lett. 142, 138 (1982). 20 L. D. Hall, G. A. Morris, and S. Sukumar, J. Am. Chem. Soc. 102, 1745 (1980). 21 R. C. Bruch and M. D. Bruch, J. Biol. Chem. 257, 3409 (1982). 22 C. C. Sweeley and H.
4 S. J. Angyal and K. James, Aust. J. Chem. 23, 1209 (1970). 15 B. Lindberg, J. L6nngren, and J. L. Thompson, Carbohydr. Res. 28, 351 (1973). 16 S. Svensson, this series, Vol. 50, p. 33. METHODS IN ENZYMOLOGY, VOL. 138 Copyright © 1987 by Academic Press, Inc. All rights of reproduction in any form reserved. ~4 The combination of methods based upon methylation analysis is especially valuable because the products (partially methylated alditol acetates or partially methylated oligosaccharides) are analyzed by mass spectrometry, 17-~9 and the amounts of material which can be analyzed (1-5/xg) 2° are therefore much smaller than the amounts required for NMR spectroscopy.
Complex Carbohydrates Part E by Victor Ginsburg (Eds.)