By C. H. W. Hirs (Eds.)
The seriously acclaimed laboratory normal, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this day - actually an important ebook for researchers in all fields of lifestyles sciences
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Extra resources for Enzyme Structure
The plates are heated for 10 minutes at 110 ° and immediately covered with a suitable glass plate that leaves an open margin for sample application, which should be started while the plate is warm. Chromatography is begun as soon as the plate has cooled down to room temperature. Cooling takes at least 20 minutes; it may be accelerated by a cooling device of the type provided in the BN chamber, for example. ~. Cellulose Layer An electric homogenizer is used to blend 8 g cellulose powder MN 300 is with 48 ml water and 2 ml ethanol for 2-3 minutes.
THIN-LAYER CHROMATOGRAPHY 45 is preferable, but adsorbent choice may be governed by other considerations. Separation, specific color tests, or substance recovery from chromatograms may be more favorable with cellulose. (Note: Adsorbents are made from rather ill-defined materials. Their quality from batch to batch is not necessarily constant. ) Instructions for Layer Preparation I. Silica Gel G Layer 1~ The silica suspension is prepared from 25 g silica gel G and 50 ml water in a closed 250-ml Erlenmeyer flask by vigorous shaking for 30 seconds.
2] MICRO M E T H O D S IN A M I N O ACID A N A L Y S I S 23 With these restrictions and limitations in mind, an apparatus in which all variables were optimized for micro determination was constructed. 11 Automatic features were retained and commercially available components were used. 5-micron spherical Dowex 50-X8 resin particles. 5 ml per hour, respectively, about the lowest practical limit of output for satisfactory operation. The pumps were supplied with synchronous motors because line voltage instability caused variable output.
Enzyme Structure by C. H. W. Hirs (Eds.)